Preparation of monolithic polymer-magnetite nanoparticle composites into poly(ethylene-co-tetrafluoroethylene) tubes for uses in micro-bore HPLC separation and extraction of phosphorylated compounds

This paper describes the fabrication of a novel microbore monolithic column modified with magnetite nanoparticles (MNPs) prepared in poly(ethylene-co-tetrafluoroethylene) (EFTE) tubing, and its application as stationary phase for chromatographic separation phosphorylated compounds. In order to obtain composite column, two-step procedure was performed. The formation glycidyl methacrylate-based monolith inside activated ETFE tube firstly carried out. Then, two incorporation approaches MNPs monoliths were investigated. generic polymer 3-aminopropyltrimethoxysilane (APTMS) be subsequently attached MNP surfaces. Alternatively, APTMS-coated used attachment onto surface through reaction epoxy groups present monolith. last strategy gave reproducible layer coated well robust permeable columns. retention behaviour this MNP-based studied by using small compounds (adenosine phosphates). It found that model analytes ruled partitioning adsorption HILIC mechanisms. columns also exhibited satisfactory performance these target compounds, showing good after months continued use. These successfully applied extraction tryptic digest β-casein.